Ebenezer Jackson, Jr., was born in Savannah, Georgia, on January 31, 1796, the son of Charlotte Fenwick and Revolutionary War veteran Ebenezer Jackson. His brother Amasa attended the United States Military Academy at West Point, New York, in the mid-1820s. After graduating from St. Mary's College in 1814, Ebenezer Jackson, Jr., studied law in Connecticut; he had a legal practice in Philadelphia, Pennsylvania, between 1821 and 1826. Jackson then moved to Middletown, Connecticut. He served in the Connecticut House of Representatives (1829-1832; 1849) and the United States House of Representatives (1834-1835). He married Hannah Saltonstall Hubbard (1815-1903) on September 1, 1840, and they had six children: Mary Selina (b. 1841), Eliza Anne (1843-1888), Robert Nesmith (1845-1915), Charles Eben (1849-1873), Katherine Frances (b. 1851), and Margaret Ellen (b. 1853). Ebenezer Jackson, Jr., died on August 17, 1874.
From the guide to the Ebenezer Jackson, Jr. papers, Jackson, Ebenezer, Jr. papers, 1672, 1814-1863, 1814-1863, (William L. Clements Library, University of Michigan)